Composite

Part:BBa_K1026000:Design

Designed by: Hongyi WU   Group: iGEM13_SJTU-BioX-Shanghai   (2013-09-16)


Constitutively Expressed dCas9 Operon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1401
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1401
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1160
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1401
    Illegal BamHI site found at 3439
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1401
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1401
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The expression level of dCas9 shall be delicately controlled, so as to acquire continuous adjustment (regulation) of target genes.


Source

The dCas9 comes from S. pyogenes.

References